Analysis of the 5'-Upstream Promoter Region of Human Involucrin Gene: Activation by 12-O-Tetradecanoylphorbol-13-Acetate
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منابع مشابه
Insulinotropic effect of the tumor promoter 12-O-tetradecanoylphorbol-13-acetate in rat pancreatic islets.
In isolated rat pancreatic islets, the tumor-promoting agent 12-O-tetradecanoylphorbol-13-acetate (TPA), when used in the 2.10(-9) to 2.10(-7) M range, was found to stimulate insulin release both in the absence and presence of glucose. The non-tumor-promoting agent 4-methylphorbol-12,13-didecanoate failed to stimulate insulin release. The insulinotropic capacity of TPA was enhanced by glucose i...
متن کاملThe Wilms' tumor suppressor, WT1, inhibits 12-O-tetradecanoylphorbol-13-acetate activation of the multidrug resistance-1 promoter.
Overexpression of P-glycoprotein, the product of the multidrug resistance-1 (MDR1) gene, is associated with treatment failure in some hematopoietic tumors. Although expression of P-glycoprotein in normal hematopoietic cells is tightly regulated during hematopoietic differentiation, its aberrant overexpression in hematopoietic malignancies occurs at the transcriptional level. We have demonstrate...
متن کاملDivergent responses in epidermal basal cells exposed to the tumor promoter 12-O-tetradecanoylphorbol-13-acetate.
Mouse epidermal basal cells can be selectively cultivated in medium with 0.02 to 0.09 mM Ca2+ and can be induced to differentiate by medium containing 1.2 mM Ca2+. Basal cell cultures were studied to determine if all cells in this population responded identically to the skin tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA). Studies on the induction of the enzyme epidermal transglutamin...
متن کاملTransient protection of cultured human cells against antitumor agents by 12-O-tetradecanoylphorbol-13-acetate.
The tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) has been shown in cell cultures to enhance the frequency of resistance to methotrexate. However, we found that TPA could also partially protect human KB cells over a short time (72 h) from the cytotoxicity of several antitumor agents, including 4'-demethylepipodophyllotoxin 9-(4,6-O-ethylidene)-beta-D-glucopyranoside (VP-16), vincris...
متن کاملGrowth of human melanocyte cultures supported by 12-O-tetradecanoylphorbol-13-acetate is mediated through protein kinase C activation.
To investigate the role of protein kinase C (PKC) in the 12-O-tetradecanoylphorbol-13-acetate (TPA)-dependent growth of human melanocytes, we analyzed the effects of phorbol ester treatment on both PKC expression and growth control in these cells. We found that established cultures of normal melanocytes contain the PKC alpha, PKC beta, and PKC epsilon isoforms. The abilities of various phorbol ...
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ژورنال
عنوان ژورنال: Journal of Investigative Dermatology
سال: 1993
ISSN: 0022-202X
DOI: 10.1111/1523-1747.ep12349867